Over the past century, lifestyle changes have led to imbalanced gut microbiota, contributing to various health issues. Probiotics offer a promising solution, but their sensitivity to oxygen, heat, and gastric acid limits their effectiveness. iNSIGHT addresses this by developing precision probiotics through a novel microencapsulation approach. The goal is to create tunable, scalable bi-gel-based microcapsules that protect probiotics and ensure their targeted delivery post-stomach. We will use a gut-on-a-chip device to study capsule breakdown and probiotic release and apply machine learning for understanding digestion kinetics and microcapsule synthesis optimization. As a proof of concept, we will demonstrate personalized probiotic treatments under pharmacy legislation. iNSIGHT paves the way for precise probiotic delivery to prevent or treat gut microbiota-related health issues.